human osteoblast media  (Cell Applications Inc)

Journal: Cellular and Molecular Bioengineering

Article Title: Remote-Controlled Gene Delivery in Coaxial 3D-Bioprinted Constructs using Ultrasound-Responsive Bioinks

doi: 10.1007/s12195-024-00818-x

Figure Lengend Snippet: Effect of varying the number of ultrasound pulses on ultrasound-controlled osteoblast cellular transfection in coaxially-bioprinted constructs. A Coaxially-bioprinted osteoblast-laden (hFOB 1.19) 4% alginate bioink containing GFP-coupled microbubbles before ultrasound, 0 hr post-ultrasound, and 48 hr post-ultrasound with varying ultrasound exposure (10, 40, or 80 pulses). B Number of transfected cells in bioprinted constructs at 48 hr post-ultrasound for varying numbers of ultrasound pulses (*p < 0.05, ****p < 0.0001, one-way ANOVA, Tukey’s multiple comparisons test, error bars denote standard deviation). C Diameter of zone containing transfected cells at 48 hr post-ultrasound for varying numbers of ultrasound pulses (one-way ANOVA, Tukey’s multiple comparisons test, error bars denote standard deviation)

Article Snippet: They were then suspended in complete media containing Human Osteoblast Media (Cell Applications, Inc.), 10% FBS (HyClone Characterized Fetal Bovine Serum CA Origin, Cytiva), and 1% pen-strep (penicillin-streptomycin, 10,000 μg/mL, Gibco) then concentrated to 3.85 × 10 7 cell/mL in complete media.

Techniques: Transfection, Construct, Standard Deviation

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: (A-D) Flow cytometric analysis was performed on (A) BM-MSC-1, (B) BM-MSC-2, (C) BM-MSC-3 and (D) BM-MSC-4. MSC surface markers CD44, CD73, CD29, and CD90 were highly expressed, whereas expression of hematopoietic stem cell marker CD45 was not detected. The percentages of cells expressing each surface marker are indicated. (E-H) Following culture of BM-MSCs in adipocyte differentiation media for 21 days, Oil Red O staining was used to detect adipocytes. Lipid droplets appear red, and nuclei appear blue. (I) BM-MSC-1 cultured in control MSC media for 21d and stained with Oil Red O. (J-M) Following exposure of BM-MSCs to osteoblast differentiation media for 30 days, von Kossa staining was used to identify osteoblasts. Bone nodules containing calcium mineral stain black. (N) BM-MSC-1 cultured in control MSC media for 30d and stained with von Kossa stain. Scale bars: 50 mm.

Article Snippet: The following day the cells were washed with PBS and media changed to human osteoblast differentiation media (Sigma-Aldrich).

Techniques: Expressing, Marker, Staining, Cell Culture

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: (A, B) Flow cytometric analysis was performed on UC-MSC-1 and UC-MSC-2. MSC surface markers CD44, CD73, CD29, and CD90 were highly expressed, whereas expression of hematopoietic stem cell marker CD45 was not detected. The percentages of cells expressing each surface marker are indicated. (C, D) Following culture of UC-MSCs in adipocyte differentiation media for 21 days, Oil Red O staining was used to detect adipocytes. Lipid droplets appear red, and nuclei appear blue. (E) UC-MSC-1 cultured in control MSC media for 21d and stained with Oil Red O. (F, G) Following exposure of UC-MSCs to osteoblast differentiation media for 30 days, von Kossa staining was used to identify osteoblasts. Bone nodules containing calcium mineral stain black. (H) U-MSC-1 cultured in control MSC media for 30d and stained with von Kossa stain. Scale bars: 50 mm.

Article Snippet: The following day the cells were washed with PBS and media changed to human osteoblast differentiation media (Sigma-Aldrich).

Techniques: Expressing, Marker, Staining, Cell Culture